Gene/Insert name:

Spp1

Alternative name:

2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;OP; Opn; Opnl; Ric; Spp-1

Plasmid description:

Lentiviral expression vector of Mus musculus secreted phosphoprotein 1(five points mutation D93G/V139A/S159G/N205S/E234K) with the Flag tag at the N-terminus and unfused CopGFP protein at the C-terminus

Species:

Mus musculus (house mouse)

Gene ID:

20750

Accessions:

NM_00120420

Vector backbone:

pLenti-CMV-GFP-Puro

Tag:

Flag(N-term);CopGFP(C-term)

Vector type:

Mammalian Expression, Lentiviral

Backbone size w/o insert:

8189bp

Cloning site 5':

Xba I

Site destroyed during cloning:

NO

Cloning site 3':

Not I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Stbl3

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Puromycin