Gene/Insert name:

Shh

Alternative name:

N/A

Plasmid description:

Mammalian Expression vector of Rattus norvegicus sonic hedgehog, fused with the EGFP protein at the C-terminus

Species:

Rattus norvegicus (Norway rat)

Gene ID:

29499

Accessions:

NM_017221

Vector backbone:

pEGFP-N1

Tag:

EGFP(C-term)

Vector type:

Mammalian Expression

Backbone size w/o insert:

4733bp

Cloning site 5':

Nhe I

Site destroyed during cloning:

NO

Cloning site 3':

Xho I

Site destroyed during cloning:

NO

Bacterial resistance:

Kanamycin

Growth strain(s):

Top10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Neomycin