Gene/Insert name:

NOS3

Alternative name:

eNOS; ECNOS

Plasmid description:

Mammalian Expression vector of Homo sapiens nitric oxide synthase 3(one point mutation T774C not causing the amino acid variation ) with C terminal 3*Flag tag

Species:

Homo sapiens (human)

Gene ID:

4846

Accessions:

NM_000603

Vector backbone:

pcDNA3.1(+)

Tag:

3*Flag(C-term)

Vector type:

Mammalian Expression

Backbone size w/o insert:

5428bp

Cloning site 5':

BamH I

Site destroyed during cloning:

NO

Cloning site 3':

Xho I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Top10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Neomycin