Gene/Insert name:

LGR5 shRNA-1

Alternative name:

FEX; HG38; GPR49; GPR67; GRP49

Plasmid description:

Mammalian shRNA Knockdown vector of Homo sapiens leucine rich repeat containing G protein-coupled receptor 5

Species:

Homo sapiens (human)

Gene ID:

8549

Accessions:

NM_003667

Vector backbone:

pPLK GFP+Puro

Tag:

GFP(N-term)

Vector type:

Lentiviral interference

Backbone size w/o insert:

8102bp

Cloning site 5':

BamH I

Site destroyed during cloning:

NO

Cloning site 3':

EcoR I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Stbl3　

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Puromycin