Gene/Insert name:

FTO(H231A/D233A)

Alternative name:

GDFD; ALKBH9; BMIQ14

Plasmid description:

Lentiviral expression of Homo sapiens FTO, alpha-ketoglutarate dependent dioxygenase(two point mutations H231A/D233A) with the AcGFP1 protein at the C-terminus

Species:

Homo sapiens (human)

Gene ID:

79068

Accessions:

NM_001080432.2

Vector backbone:

pLVX-AcGFP1-N1

Tag:

AcGFP1(C-term)

Vector type:

Mammalian Expression, Lentiviral

Backbone size w/o insert:

8834bp

Cloning site 5':

Xho I

Site destroyed during cloning:

NO

Cloning site 3':

Sma I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Stbl3

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Puromycin