Gene/Insert name:

Cdkn1b

Alternative name:

p27; Kip1; p27Kip1; AA408329; AI843786

Plasmid description:

Mammalian Expression vector of Mus musculus cyclin-dependent kinase inhibitor 1B with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus

Species:

Mus musculus (house mouse)

Gene ID:

12576

Accessions:

NM_009875.4

Vector backbone:

pIRES2-EGFP

Tag:

3*Flag(N-term);EGFP(C-term)

Vector type:

Mammalian Expression

Backbone size w/o insert:

5300bp

Cloning site 5':

Xho I

Site destroyed during cloning:

NO

Cloning site 3':

EcoR I

Site destroyed during cloning:

NO

Bacterial resistance:

Kanamycin

Growth strain(s):

Top10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Neomycin