Gene/Insert name:

BRAF

Alternative name:

NS7; B-raf; BRAF1; RAFB1; B-RAF1

Plasmid description:

Lentiviral expression of Homo sapiens B-Raf proto-oncogene, serine/threonine kinase, fused with the CopGFP protein at the C-terminus

Species:

Homo sapiens (human)

Gene ID:

673

Accessions:

NM_004333.4

Vector backbone:

pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro

Tag:

CopGFP(C-term)

Vector type:

Mammalian Expression, Lentiviral

Backbone size w/o insert:

8189bp

Cloning site 5':

Xba I

Site destroyed during cloning:

NO

Cloning site 3':

Not I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Stbl3

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Puromycin