Gene/Insert name:

Beclin1(E186G)

Alternative name:

BECN1,ATG6,VPS30

Plasmid description:

Lentiviral expression vector of Homo sapiens beclin 1(one point mutation E186G) with unfused CopGFP protein at the C-terminus.Identical with the NCBI sequence except for one point mutation: 837 T/C not causing the amino acid variation.

Species:

Homo sapiens (human)

Gene ID:

8678

Accessions:

NM_003766.3

Vector backbone:

pLenti-CMV-GFP-Puro

Tag:

CopGFP(C-term)

Vector type:

Mammalian Expression, Lentiviral

Backbone size w/o insert:

8189bp

Cloning site 5':

EcoR I

Site destroyed during cloning:

NO

Cloning site 3':

BamH I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Stbl3

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Puromycin