Gene/Insert name:

Alox12

Alternative name:

9930022G08Rik; Alox12p; P-12LO

Plasmid description:

Mammalian Expression vector of Mus musculus arachidonate 12-lipoxygenase with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus

Species:

Mus musculus (house mouse)

Gene ID:

11684

Accessions:

NM_007440

Vector backbone:

pIRES2-EGFP

Tag:

3*Flag(N-term);EGFP(C-term)

Vector type:

Mammalian Expression

Backbone size w/o insert:

5300bp

Cloning site 5':

Xho I

Site destroyed during cloning:

NO

Cloning site 3':

Sal I

Site destroyed during cloning:

NO

Bacterial resistance:

Kanamycin

Growth strain(s):

Top10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Neomycin