Gene/Insert name:

AKT1(T308D/S473D)

Alternative name:

AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC;RAC-ALPHA

Plasmid description:

Mammalian Expression vector of Homo sapiens AKT serine/threonine kinase 1(two points mutation T308D/S473D) with N terminal Flag tag

Species:

Homo sapiens (human)

Gene ID:

207

Accessions:

NM_005163

Vector backbone:

pcDNA3.1(+)

Tag:

Flag(N-term)

Vector type:

Mammalian Expression

Backbone size w/o insert:

5428bp

Cloning site 5':

Kpn I

Site destroyed during cloning:

NO

Cloning site 3':

EcoR I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Top10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Neomycin