Gene/Insert name:

WRN

Alternative name:

RECQ3; RECQL2; RECQL3

Plasmid description:

Mammalian Expression vector of Homo sapiens WRN RecQ like helicase(three point mutations G252A/C513T/C4083T not causing the amino acid variation) with N terminal 3xHA tag

Species:

Homo sapiens (human)

Gene ID:

7486

Accessions:

NM_000553.6

Vector backbone:

pcDNA3.1(+)

Tag:

3*HA(N-term)

Vector type:

Mammalian Expression

Backbone size w/o insert:

5428bp

Cloning site 5':

BamH I

Site destroyed during cloning:

NO

Cloning site 3':

EcoR I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Top 10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Neomycin