Gene/Insert name:

Capn1

Alternative name:

N/A

Plasmid description:

Lentiviral expression vector of Rattus norvegicus calpain 1(Identical with the NCBI sequence except for three point mutations: T489C/G1659A/A1872C not causing the amino acid variation) with the unfused CopGFP protein at the C-terminus

Species:

Rattus norvegicus (Norway rat)

Gene ID:

29153

Accessions:

NM_019152.2

Vector backbone:

pLenti-CMV-GFP-Puro

Tag:

CopGFP(C-term)

Vector type:

Mammalian Expression, Lentiviral

Backbone size w/o insert:

8189bp

Cloning site 5':

EcoR I

Site destroyed during cloning:

NO

Cloning site 3':

Swa I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Stbl3

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Puromycin