Gene/Insert name:

Traf3 sgRNA

Alternative name:

AI528849; amn; CAP-1; CD40bp; CRAF1; LAP1;T-BAM; TRAFAMN

Plasmid description:

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting Mus musculus TNF receptor-associated factor 3

Species:

Mus musculus (house mouse)

Gene ID:

22031

Accessions:

NM_011632.3

Vector backbone:

pSpCas9(BB)-2A-GFP (PX458)

Tag:

3*Flag(N-term);2A-EGFP(C-term)

Vector type:

CRISPR

Backbone size w/o insert:

9289bp

Cloning site 5':

Bbs I

Site destroyed during cloning:

Yes

Cloning site 3':

Bbs I

Site destroyed during cloning:

Yes

Bacterial resistance:

Ampicillin

Growth strain(s):

Top10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

None