Gene/Insert name:

ITGB1 sgRNA

Alternative name:

CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA

Plasmid description:

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting Homo sapiens integrin subunit beta 1

Species:

Homo sapiens (human)

Gene ID:

3688

Accessions:

N/A

Vector backbone:

pSpCas9(BB)-2A-GFP (PX458)

Tag:

3*Flag(N-term);2A-EGFP(C-term)

Vector type:

CRISPR

Backbone size w/o insert:

9288bp

Cloning site 5':

Bbs I

Site destroyed during cloning:

Yes

Cloning site 3':

Bbs I

Site destroyed during cloning:

Yes

Bacterial resistance:

Ampicillin

Growth strain(s):

Top10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

None