Bioworld Technology

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RecombinantMCP-5/CCL12,Mouse BK0266

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Specifications

size	10μg / 50μg / 1mg

Source:

CHO

Molecular Weight:

9.3-11 kDa, observed by reducing SDS-PAGE.

Purity:

> 98% as analyzed by SDS-PAGE.

Biological activity:

The EC50 value of mouse MCP-5/CCL12 on Caˆ2+ mobilization assay in CHO-K1/G15/mCCR2 cells (human G15 and mouse CCR2 stably expressed in CHO-K1 cells) is less than 0.5 μg/ml.

AA Sequence:

GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWV KNSINHLDKTSQTFILEPSCLG

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Monocyte Chemotactic Protein-5(MCP-5), also known as Chemokine (C-C motif) ligand 12(CCL12), is a small cytokine belonging to the CC chemokine family that has been characterized in mice. Due to its similarity with human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. MCP-5 specifically attracts eosinophils, monocytes and lymphocytes.It can signal through the CCR2 receptor.Recombinant Mouse MCP-5/CCL1 produced in CHO cells is a polypeptide chain containing 82 amino acids. A fully biologically active molecule, rmMCP-5/CCL12 has a molecular mass of 11 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O or PBS at 100 μg/ml.

Storage:

Lyophilized recombinant Mouse MCP-5/CCL12 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse MCP-5/CCL12 should be stable up to 1 week at 4°C or up to 2 months at -20°C.