Source:

Escherichia coli.

Molecular Weight:

24.5 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% as analyzed by SDS-PAGE and HPLC.

Biological activity:

ED50 < 10 ng/mL, measured by a cell proliferation assay using Rat Embryo Brain cells, corresponding to a specific activity of > 1× 10ˆ5 units/mg.

AA Sequence:

MKRGCSSSS PKLLSQLKSQ ANITGNTASL LEPYILHQNL NTLTLRAACT EHPVAFPSED MLRQLSKPDF LSTVHATLGR VWHQLGAFRQ QFPKIQDFPE LERARQNIQG IRNNVYCMAR LLHPPLEIPE PTQADSGTSR PTTTAPGIFQ IKIDSCRFLW GYHRFMGSVG RVFEEWGDGS RRSRRHSPLW AWLKGDHRIR PSRSSQSAML RSLVPR

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, which also includes IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and shares a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells. Recombinant Rat Oncostatin M (rrOSM) produced in E.coli is a single non-glycosylated polypeptide chain containing 215 amino acids. A fully biologically active molecule, rrOSM has a molecular mass of 24.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O or PBS at 100 μg/ml.

Storage:

Lyophilized recombinant Rat Oncostatin M (rrOSM) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrOSM remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.