Bioworld Technology

Bioworld Technology is manufacturer of over 15,000 highly purified Monoclonal and Polyclonal Antibodies, Peptides, Proteins, and other related Research Products. Their products are used in all biological fields. An important focus in research taking place today is in work investigating Signal transduction pathways, Cardiac markers, Neuroscience as well as Stem cell research. Bioworld Technology is committed to providing customers with innovative research tools and to helping scientists determine the mechanisms of cell function and disease.
Bioworld Technology is one of the leading phospho-antibody manufacturers. They have produced over 500 phospho-antibodies for AKT, AMPK, GSK, STAT pathways. The peptides corresponding to each phospho-antibody have also been listed to help scientists.
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RecombinantNT-4,Mouse BK0145

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Specifications

size	10μg / 50μg / 1mg

Source:

Escherichia coli.

Molecular Weight:

14.0 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% by SDS-PAGEanalysis.

Biological activity:

ED50 <1µg/mL, measured by a cell proliferation assay usingC6cells, corresponding to a specific activity of>1× 10ˆ3 units/mg.

AA Sequence:

MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKAESAGE GGPGVGGGGC RGVDRRHWLS ECKAKQSYVR ALTADSQGRV GWRWIRIDTA CVCTLLSRTG RA

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against 50mM acetic acid.

Background:

Neurotrophin-4 (NT-4) is a small secreted cytokine, and belongs to the Neurotrophin (NT) family, which also includes Brain Derived Neurotropic Factor (BDNF), Nerve Growth Factor (NGF), and NT-3. NT family members are all derived from similar sized protein precursors, composed of N-terminal propeptides and C-terminal mature domains, which are separated by posttranslational proteolytic cleavage. NT-4 (along with NT-3) is foundin the brains of mammals . In vivo, NT-4 binds to the common receptor, p75NTR, and a tyrosine kinase receptor, TrkB. The heterotrimeric complex activates the NFκB transcription factor. NT-4 is essential for the differentiation and wiring regulation of the central and peripheral nervous systems during development, and is related to important diseases including Alzheimer’s.Recombinant mouse Neurotrophin-4 (rmNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chainsof 131 amino acids. A fully biologically active molecule, rmNT-4 has a molecular mass of 14.0kDaanalyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in 50mM acetic acid or ddH2O at 100 μg/mL.

Storage:

Lyophilized recombinant mouse Neurotrophin-4 (rmNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmNT-4 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.