Bioworld Technology

Bioworld Technology is manufacturer of over 15,000 highly purified Monoclonal and Polyclonal Antibodies, Peptides, Proteins, and other related Research Products. Their products are used in all biological fields. An important focus in research taking place today is in work investigating Signal transduction pathways, Cardiac markers, Neuroscience as well as Stem cell research. Bioworld Technology is committed to providing customers with innovative research tools and to helping scientists determine the mechanisms of cell function and disease.
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RecombinantM-CSF,Human BK0127

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Specifications

size	10μg / 100μg / 1mg

Source:

Escherichia coli.

Molecular Weight:

28 kDa, observed by non-reducing SDS-PAGE.

Purity:

> 95% as analyzed by non-reducing SDS-PAGE.

Biological activity:

ED50 of 1 - 3 ng/ml, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 10ˆ5-1 x 10ˆ6 units/mg.

AA Sequence:

MEEVSEYCS HMIGSGHLQS LQRLIDSQME TSCQITFEFV DQEQLKDPVC YLKKAFLLVQ DIMEDTMRFR DNTPNAIAIV QLQELSLRLK SCFTKDYEEH DKACVRTFYE TPLQLLEKVK NVFNETKNLL DKDWNIFSKN CNNSFAECSS QGHERQSEGS

Endotoxin:

<1 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.

Background:

Macrophage Colony-Stimulating Factor 1 (M-CSF), involved especially in monocytopoiesis,[1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63.Recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O or PBS or Tris-HCl, pH 8.0 at 100 μg/ml.

Storage:

Lyophilized recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhM-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.