RecombinantLR3-IGF-I(ReceptorGrade),Human BK0123
Specifications
| 100μg / 1mg |
Source:
Escherichia coli.
Molecular Weight:
9.1±0.9 KDa, observed by reducing SDS-PAGE.
Purity:
> 95% by SDS-PAGE.
Biological activity:
ED50 ＜10 ng/ml, measured by the dose-dependantproliferation of CHO cells, corresponding to a specific activity of >1.0× 10ˆ5 units/mg.
AA Sequence:
MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
Endotoxin:
＜1 EU/μg, determined by LAL method.
Formulation:
Lyophilized after dissolved in 48%acetonitrile and 0.1% TFA
Background:
IGF-1 is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-1 is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-2, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-1 (rhLR3IGF-1) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3)and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs. RhLR3IGF-1 is properly folded under oxidizing conditions and then purified by proprietary chromatographic techniques at GenScript.
Usage:
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Reconstitution:
Reconstituted in 10mM HCl at 1mg/ml. Use a 0.22 μm membrane for filter Sterilization.
Storage:
Lyophilized recombinant human Long R3Insulin-like Growth Factor-1 (rhLR3IGF-1) remains stable up to 6 months at -80°C from date of receipt. Stock solution of peptide can be stored at least 3 months at -20°C. Avoid repeated freeze-thaw cycles.