Bioworld Technology

Bioworld Technology is manufacturer of over 15,000 highly purified Monoclonal and Polyclonal Antibodies, Peptides, Proteins, and other related Research Products. Their products are used in all biological fields. An important focus in research taking place today is in work investigating Signal transduction pathways, Cardiac markers, Neuroscience as well as Stem cell research. Bioworld Technology is committed to providing customers with innovative research tools and to helping scientists determine the mechanisms of cell function and disease.
Bioworld Technology is one of the leading phospho-antibody manufacturers. They have produced over 500 phospho-antibodies for AKT, AMPK, GSK, STAT pathways. The peptides corresponding to each phospho-antibody have also been listed to help scientists.
All of their high quality antibodies, peptides and kits were tested in their own facility with original published pictures included. They have a commitment to customer service and offer 100% quality satisfaction. If our products do not match the results listed on the data sheet, we will help you to troubleshoot or refund for the full credit.
Bioworld Technology continues to manufacture product lines to the highest standards; and all of their scientists are dedicated to the mystery of the world of science.

RecombinantKGF/FGF-7,Mouse BK0117

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Specifications

size	10μg / 50μg / 1mg

Source:

Escherichia coli.

Molecular Weight:

18.7 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% by SDS-PAGE and HPLC analyses.

Biological activity:

ED50 < 2 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0× 10ˆ5 units/mg.

AA Sequence:

CND MSPEQTATSV NCSSPERHTR SYDYMEGGDI RVRRLFCRTQ WYLRIDKRGK VKGTQEMKNS YNIMEIRTVA VGIVAIKGVE SEYYLAMNKE GKLYAKKECN EDCNFKELIL ENHYNTYASA KWTHSGGEMF VALNQKGIPV KGKKTKKEQK TAHFLPMAIT

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of the FGF receptor, FGFR2-IIIb. FGFR2-IIIb is expressedby most epithelial cells, indicating KGF’s roleas a paracrine mediator. KGF induces the differentiation and proliferation of various epithelial cells such as keratinocytes in the epidermis, hair follicles and sebaceous glands., KGF is also responsible for wound repair of various tissuesincluding lung, bladder, and kidney. Recombinant mouse Keratinocyte Growth Factor (rmKGF) produced in E.coli is a single non-glycosylated polypeptide chain containing 163 amino acids. A fully biologically active molecule, rmKGF has a molecular mass of 18.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in PBS at 100 μg/ml.

Storage:

Lyophilized recombinant mouse Keratinocyte Growth Factor (rmKGF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmKGF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.