Source:

Escherichia coli.

Molecular Weight:

15.5 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% as analyzed by SDS-PAGE and HPLC.

Biological activity:

ED50 <0.5 ng/ml, measured by cytotoxicity assay using WEHI-279 cells, corresponding to a specific activity of >2×10ˆ6 units/mg.

AA Sequence:

GT LIESLESLKN YFNSSSMDAM EGKSLLLDIW RNWQKDGNTK ILESQIISFY LRLFEVLKDN QAISNNISVI ESHLITNFFS NSKAKKDAFM SIAKFEVNNP QIQHKAVNEL IRVIHQLSPE SSLRKRKRSR C

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Interferon gamma (IFN-γ), also known as Type II interferon,is a cytokine produced primarily by T-lymphocytes and natural killer cells. The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and activates the IFN-γ/JAK/STAT pathway. IFN-γ signaling promotesbiological functions primarily related to antiviral and antibacterial defense, apoptosis, inflammation, and regulation of innate and acquired immune responses. While IFN-γ–induced inflammatory cascades summon a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.Recombinant Rat Interferon gamma (IFN-γ) produced in E.coli is a single non-glycosylated polypeptide chain containing 134 amino acids. A fully biologically active molecule, rrIFN-γ has a molecular mass of 15.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O or PBS at 100 μg/ml.

Storage:

Lyophilized recombinant Rat IFN-γ remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant Rat IFN-γ should be stable up to 1 week at 4°C or up to 2 months at -20°C.