RecombinantHCC-1/CCL14(72aa),Human BK0074
Specifications
| 5μg / 25μg / 1mg |
Source:
Escherichia coli.
Molecular Weight:
8.4 kDa, observed by reducing SDS-PAGE.
Purity:
> 95% by SDS-PAGE analysis.
Biological activity:
ED50 < 25 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL14, corresponding to a specific activity of > 40 units/mg.
AA Sequence:
TES SSRGPYHPSE CCFTYTTYKI PRQRIMDYYE TNSQCSKPGI VFITKRGHSV CTNPSDKWVQ DYIKDMKEN
Endotoxin:
< 0.2 EU/μg, determined by LAL method.
Formulation:
Lyophilized after extensive dialysis against PBS.
Background:
HCC-1/CCL14 is a member of the chemokine family, which are small chemotactic proteins that regulate cell migration under inflammatory and steady state conditions. HCC-1 is expressed in epithelial and decidual cells and is unique among chemokines due to its high abundance in normal human plasma. HCC-1 can bind to chemokine receptors CCR1 and CCR5, however full length HCC-1 is a weak agonist of CCR1 and only becomes potent after removal of its eight N-terminal residues. Chemokine decoy receptor D6 can bind HCC-1 and promote its degradation as a means to regulate its level in vivo. Functionally HCC-1 promotes trophoblast migration by regulating extracellular matrix components as well as specific adhesion molecules.Recombinant human Hemofiltrate CC Chemokine-1 (72 a.a.) (HCC-1)/CCL14 (rhHCC-1) produced in E.coli is a single non-glycosylated polypeptide chain containing 72 amino acids. A fully biologically active molecule, rhHCC-1 has a molecular mass of 8.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Usage:
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Reconstitution:
Reconstituted in ddH2O at 100 μg/mL.
Storage:
Lyophilized recombinant human Hemofiltrate CC Chemokine-1 (72 a.a.) (HCC-1)/CCL14 (rhHCC-1) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhHCC-1 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.