Source:

Escherichia coli.

Molecular Weight:

21.9kDa, observed by reducing SDS-PAGE.

Purity:

> 95% by SDS-PAGE and HPLC analyses

Biological activity:

Measured by its ability to induce cell proliferation of RPMI 8226 Cells.

AA Sequence:

MRREVSRLQ RSGGPSQKQG ERPWQSLWEQ SPDVLEAWKD GAKSRRRRAV LTQKHKKKHS VLHLVPVNIT SKDSDVTEVM WQPVLRRGRG LEAQGDIVRV WDTGIYLLYS QVLFHDVTFT MGQVVSREGQ GRRETLFRCI RSMPSDPDRA YNSCYSAGVF HLHQGDIITV KIPRANAKLS LSPHGTFLGF VKL

Endotoxin:

<0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against 20mM acetic acid.

Background:

A Proliferation-inducing Ligand (April)) also known as TNSF13A, Tall-2, and TRDL-1, is a member of the TNF ligand (TNFL) superfamily. April is most similar to B-cell activation factor (BAFF) with which it shares 30% sequence identity, compete for two receptors, TACI and BCMA. APRIL is expressed at low levels in lymphoid tissue and is over-expressed by a number of tumors. April has a proliferative effect on both normal and tumor cell lines in vitro and in vivo. APRIL seems to be involved in the regulation of death and proliferation of tumor cells, but there are still contradictory findings regarding its overall biological effects. Recombinant mouse A Proliferation-inducing Ligand (rmApril) produced in E. coli is a single non-glycosylated polypeptide chain containing 192 amino acids. A fully biologically active molecule, rmApril has a molecular mass of 21.9kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O at 100 μg/ml.

Storage:

Lyophilized recombinant mouse A Proliferation-inducing Ligand (rmApril) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmApril should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.