Alternative names:

Host:

His-tag

Immunogen:

Reactivity:

E. coli Expression

Description:

Inclusion body;0.5mol Urea,PH=8

Specificity:

Carrier:pET30a-KFDV-E-His(C-term) Virus:Kyasanur Forest disease virus E protein

Bio-MW:

288bp;11kDa

Applications:

Reseach

Application dilution:

Reseach

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Kyasanur Forest Disease (KFD) is a tick-borne viral hemorrhagic disease caused by the Kyasanur Forest Disease Virus (KFDV). The virus is a member of family Flaviviridae, genus flavivirus and categorized as a bio-safety class-4 level pathogen1. KFDV was first identified and isolated from sick and dead monkeys in the forest regions of Shimoga district situated in the State of Karnataka, India and reported during the year 19572. Eventually, KFD infections were noticed among persons who visited the forests. Monkeys and humans are most susceptible to the KFDV, and the virus gets transmitted through hard ticks belonging to the genus Haemaphysalis. Since the discovery of KFDV, the disease was investigated in and around Karnataka state and it was observed that the KFDV was reported from only five districts of Karnataka namely, Shimoga, Chikkamagalore, Uttara-Kannada, Dakshina-Kannada, and Udupi4.

Note:

For research use only, not for use in diagnostic procedure.