Specifity:

Di-Methyl-Histone H3 (K10) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when di-methylated at K10.

Alternative names:

HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/

Species reactivity:

Human

Source:

Polyclonal antibody preparation

Host:

Rabbit

Ig type:

IgG

Immunogen:

Synthesized peptide derived from the N-terminal region of human Histone H3 around the di-methylation site of K10.

Purification:

Rabbit Polyclonal IgG, affinity purified by Protein A.

Concentration:

1 mg/mL

Purify:

≥90% by SDS-PAGE

Molecular weight:

17 kDa

Form:

Lyophilized or Liquid

Applications:

WB; IHC-p; ELISA

Usage:

WB 1:500-2000; IHC-p 1:100-300; ELISA 1:10000; Not yet tested in other applications.

Storage buffer:

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage:

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.

Precautions:

This product is for research use only, not for diagnostic or therapeutic use.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com