Specifity:

Phospho-Smad3 (S425) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S425.

Alternative names:

SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Species reactivity:

Human

Source:

Polyclonal antibody preparation

Host:

Rabbit

Ig type:

IgG

Immunogen:

Synthesized peptide derived from human Smad3 around the phosphorylation site of S425.

Purification:

Rabbit Polyclonal IgG, affinity purified by Protein A.

Concentration:

1 mg/mL

Purify:

≥90% by SDS-PAGE

Molecular weight:

58 kDa

Form:

Lyophilized or Liquid

Applications:

WB; IHC; ELISA

Usage:

WB 1:500-2000; IHC 1:100-300; ELISA 1:40000; Not yet tested in other applications.

Storage buffer:

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage:

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.

Precautions:

This product is for research use only, not for diagnostic or therapeutic use.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com