Product info:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Alternative names:

FO108; H4; H4/n; H4F2; H4FN; HIST2H4; Histone H4; HIST1H4A; HIST2H4A

Species reactivity:

Human, Mouse, Rat, Other (Wide Range)

Host:

Rabbit

Cellular localisation:

Chromosome, Nucleus

Isotype:

IgG

Immunogen:

A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4

Positive control:

HeLa, NIH/3T3, C6

AA Sequence:

Email info@sobekbio.com for sequence

Purification:

Affinity purification

Molecular weight:

Calculated MW: 11kDa Observed MW: 11KDa

Form:

Liquid

Applications:

IF (HeLa) WB (Mouse uterus, Homo sapiens, Mus musculus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIP-seq 1:20 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com