Phospho-CDK1-Y15 Rabbit pAb (AMR00774N) AMR00774N

Product info:

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternative names:

CDK1; CDC2; CDC28A; P34CDC2

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around Y15 of human CDK1 (NP_001777.1).

Positive control:

HT-29, HeLa

AA Sequence:

GTYGV

Purification:

Affinity purification

Molecular weight:

Calculated MW: 27kDa/34kDa Observed MW: 34kDa

Form:

Liquid

Applications:

WB (H460, A549, HeLa, Mus musculus, Homo sapiens, Eriocheir sinensis) IF (Mus musculus, Homo sapiens) IP (Homo sapiens)

Dilution:

WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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