Phospho-Histone H2AX-S139 Rabbit pAb (AMR00845N) AMR00845N

Product info:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Alternative names:

H2A.X; H2A/X; H2AX; Histone H2AX; H2AFX; histone H2AX; gamma H2A.X; γH2AX

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Chromosome, Nucleus

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1).

Positive control:

NIH/3T3, C6

AA Sequence:

TQASQ

Purification:

Affinity purification

Molecular weight:

Calculated MW: 15kDa Observed MW: 15KDa

Form:

Liquid

Applications:

WB (U251 cells, N2A, Homo sapiens, Mus musculus) IF (Mouse bone marrow, Homo sapiens, Mus musculus) ChIP () IHC (Homo sapiens)

Dilution:

WB 1:500 - 1:1000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com