Acetyl-Histone H3-K14 Rabbit pAb (AMR00752N) AMR00752N

Product info:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Alternative names:

H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A

Species reactivity:

Human, Mouse, Rat, Other (Wide Range)

Host:

Rabbit

Cellular localisation:

Chromosome, Nucleus

Isotype:

IgG

Immunogen:

A synthetic acetylated peptide corresponding to residues surrounding K14 of human H3

Positive control:

HeLa, NIH/3T3, C6

AA Sequence:

Email info@sobekbio.com for sequence

Purification:

Affinity purification

Molecular weight:

Calculated MW: 15kDa Observed MW: 17KDa

Form:

Liquid

Applications:

WB (HL-60, Mus musculus, Rattus norvegicus) IP (Rattus norvegicus) ChIP (Homo sapiens, Rattus norvegicus, Mus musculus) ChIP-qPCR (Rattus norvegicus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com