VEGF Mouse mAb (AMM08801N) AMM08801N

Product info:

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

Alternative names:

VEGFA; MVCD1; VEGF; VPF; L VEGFA; VEGF A

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Cellular localisation:

Secreted

Isotype:

IgG

Immunogen:

Recombinant protein of human VEGFA

Positive control:

Mouse heart, Mouse kidney, Mouse lung, Rat heart, Rat kidney, Rat lung

AA Sequence:

Email info@sobekbio.com for sequence

Purification:

Affinity purification

Molecular weight:

Calculated MW: 15-27kDa/34-45kDa Observed MW: 20kDa

Form:

Liquid

Applications:

WB (Homo sapiens) IHC (Homo sapiens, Mus musculus) ELISA (Mus musculus)

Dilution:

WB 1:500 - 1:1000 IHC 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com