Phospho-ULK1-S757 Rabbit pAb (AMR01039N) AMR01039N

Product info:

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1 via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. Phosphorylates FLCN, promoting autophagy. Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation.

Alternative names:

ULK1; ATG1; ATG1A; UNC51; Unc51.1; hATG1; ULk1

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Cytoplasm, Preautophagosomal structure, cytosol

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around S757 of human Phospho-ULK1-S757 (NP_003556.1).

Positive control:

A-431

AA Sequence:

TVGSP

Purification:

Affinity purification

Molecular weight:

Calculated MW: 150kDa Observed MW: 145KDa

Form:

Liquid

Applications:

WB (Homo sapiens, Spodoptera frugiperda) IHC (Homo sapiens)

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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