phospho-LATS1-S909/LATS2-S872 Rabbit pAb (AMR01174N) AMR01174N

Product info:

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Alternative names:

LATS1; WARTS; wts

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Cytoplasm, centrosome, cytoskeleton, microtubule organizing center

Isotype:

IgG

Immunogen:

A phospho specific peptide corresponding to residues surrounding S909 of human LATS1.

Positive control:

HeLa

AA Sequence:

Email info@sobekbio.com for sequence

Purification:

Affinity purification

Molecular weight:

Calculated MW: 76kDa/126kDa Observed MW: 126kDa

Form:

Liquid

Applications:

WB (Homo sapiens, Rattus norvegicus)

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com