Phospho-PRKACA-S339 Rabbit pAb (AMR00990N) AMR00990N

Product info:

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Alternative names:

PRKACA; PKACA; PPNAD4

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Cell membrane, Cell projection, Cytoplasm, Lipid-anchor, Membrane, Mitochondrion, Nucleus, cilium, flagellum

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around S339 of human PRKACA (NP_002721.1).

Positive control:

HeLa, 293

AA Sequence:

RVSIN

Purification:

Affinity purification

Molecular weight:

Calculated MW: 39kDa/40kDa Observed MW: 42kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com