Product info:

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Alternative names:

ZAP70; ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Cell membrane, Cytoplasm, Peripheral membrane protein

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around Y319 of human ZAP70 (NP_001070.2).

Positive control:

Jurkat, Preincubated with blocking peptide

AA Sequence:

SPYSD

Purification:

Affinity purification

Molecular weight:

Calculated MW: 35kDa/55kDa/69kDa Observed MW: 70kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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