Phospho-Chk1-S345 Rabbit pAb (AMR01006N) AMR01006N

Product info:

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Alternative names:

CHEK1; CHK1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around S345 of human Chk1 (NP_001265.2).

Positive control:

HT-29, HepG2, C6

AA Sequence:

SFSQP

Purification:

Affinity purification

Molecular weight:

Calculated MW: 43kDa/50kDa/54kDa Observed MW: 54kDa

Form:

Liquid

Applications:

WB (Human lung cancer cell, H460, Mus musculus, Homo sapiens)

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com