Product info:

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Alternative names:

NFKB3; p65; NF-kB p65; RELA; CMCU

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Nucleus

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human NF-kB p65/RelA (NP_068810.3).

Positive control:

HeLa, 293T, MCF7, Mouse lung, Mouse kidney, Rat lung, Rat kidney

AA Sequence:

RSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLS

Purification:

Affinity purification

Molecular weight:

Calculated MW: 58kDa/59kDa/60kDa Observed MW: 65KDa

Form:

Liquid

Applications:

WB (RAW264.7, Mouse atherosclerosis arterial tissue, HCC827, U251, Mus musculus, Homo sapiens, Rattus norvegicus, Schisandra chinensis, Gallus gallus, Macaca mulatta, Sus scrofa, ?Cryptosporidium parvum, Alpinia katsumadai Hayata) IF (Rat H9c2 cells, Mus musculus, Homo sapiens, Rattus norvegicus) ChIP (Homo sapiens) IHC (Mus musculus, Rattus norvegicus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com