Product info:

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

Alternative names:

TSG10; VPS23; TSG101 / VPS23; TSG101

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Late endosome membrane, Membrane, Nucleus, Peripheral membrane protein

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TSG101/VPS23 (NP_006283.1).

Positive control:

HeLa, MCF7, K-562, Mouse spinal cord, Rat heart, Rat lung

AA Sequence:

SALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY

Purification:

Affinity purification

Molecular weight:

Calculated MW: 31kDa/43kDa Observed MW: 44kDa

Form:

Liquid

Applications:

WB (HEK293T, Homo sapiens, Mus musculus, Rattus norvegicus, Cynoglossus semilaevis, Gallus gallus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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