Product info:

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Alternative names:

IME4; M6A; MT-A70; Spo8; hMETTL3; METTL3

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Nucleus speckle

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).

Positive control:

293T, HT-1080, NIH/3T3, Mouse testis, Mouse brain, Rat spleen

AA Sequence:

MSDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAM

Purification:

Affinity purification

Molecular weight:

Calculated MW: 25kDa/64kDa Observed MW: 70KDa

Form:

Liquid

Applications:

WB (Human breast cancer, HeLa, Homo sapiens, Mus musculus, Rattus norvegicus) IF (Human breast cancer, Homo sapiens, Mus musculus) RIP (Homo sapiens) Co-IP (Homo sapiens) IHC (Mus musculus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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