Product info:

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

Alternative names:

MLKL; hMLKL

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Cell membrane, Cytoplasm

Isotype:

IgG

Immunogen:

Recombinant protein of mouse MLKL

Positive control:

HeLa, Jurkat, HT-1080, NCI-H460

AA Sequence:

Email info@sobekbio.com for sequence

Purification:

Affinity purification

Molecular weight:

Calculated MW: 53kDa/54kDa Observed MW: 54kDa

Form:

Liquid

Applications:

WB (human kidney cancer cells, Mus musculus, Ctenopharyngodon idellus, Homo sapiens, Rattus norvegicus, Sus scrofa) IHC (Mus musculus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com