Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein(CRP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C Reactive Protein(CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C Reactive Protein(CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C Reactive Protein(CRP) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Species reactivity:
Cat
Alternative names:
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Sample type:
Serum, plasma and other biological fluids.
Detection range:
Sensitivity:
7.4 pg/mL
Standard:
1000 pg/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com