Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin(b2M). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-2-Microglobulin(b2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin(b2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-2-Microglobulin(b2M) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Infection immunity;Kidney biomarker;
Species reactivity:
Simian
Alternative names:
Beta 2M; BMG; B2MG
Sample type:
Serum, plasma, urine and other biological fluids
Detection range:
Sensitivity:
1.19 ng/mL
Standard:
200 ng/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com