Product info:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ghrelin(GHRL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ghrelin(GHRL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ghrelin(GHRL) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Signal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;
Species reactivity:
Rabbit
Alternative names:
MTLRP; Growth Hormone-Releasing Peptide
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range:
Sensitivity:
49.2 pg/mL
Standard:
10000 pg/mL
Detection method:
Competitive Inhibition
Assay time:
2h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com