Product info:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cross Linked C-Telopeptide Of Type III Collagen(CTXIII) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cross Linked C-Telopeptide Of Type III Collagen(CTXIII). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cross Linked C-Telopeptide Of Type III Collagen(CTXIII) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Metabolic pathway;Nutrition metabolism;Bone metabolism;
Species reactivity:
Dog
Alternative names:
CTX-III; CTX3
Sample type:
Serum, plasma and other biological fluids
Detection range:
Sensitivity:
46.8 pg/mL
Standard:
10000 pg/mL
Detection method:
Competitive Inhibition
Assay time:
2h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com