Product info:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Beta-Hydroxybutyric Acid(bHB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-Hydroxybutyric Acid(bHB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Hydroxybutyric Acid(bHB) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Signal transduction;Metabolic pathway;Endocrinology;
Species reactivity:
Avian
Alternative names:
OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range:
Sensitivity:
0.48 μg/mL
Standard:
100 μg/mL
Detection method:
Competitive Inhibition
Assay time:
2h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com