Product info:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dopamine(DA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dopamine(DA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine(DA) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Signal transduction;Cardiovascular biology;Reproductive science;Neuro science;
Species reactivity:
Avian
Alternative names:
2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride
Sample type:
serum, plasma and other biological fluids
Detection range:
Sensitivity:
4.71 pg/mL
Standard:
1000 pg/mL
Detection method:
Competitive Inhibition
Assay time:
2h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com