Product info:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cortisol(Cor) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cortisol(Cor). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cortisol(Cor) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Metabolic pathway;Endocrinology;Hormone metabolism;
Species reactivity:
Cattle
Alternative names:
Hydrocortisone
Sample type:
serum, plasma, urine and other biological fluids
Detection range:
Sensitivity:
0.93 ng/mL
Standard:
200 ng/mL
Detection method:
Competitive Inhibition
Assay time:
2h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com