Product info:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cr(Creatinine) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cr(Creatinine). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cr(Creatinine) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Tumor immunity;Neuro science
Species reactivity:
Rabbit
Alternative names:
CR; Calretinin; CALB2; CAB29; CAL2; 29 kDa calbindin; calbindin 2; calbindin 2;; 29kD; calretinin; calbindin D29K
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection range:
Sensitivity:
0.75 nmol/ml
Standard:
80 nmol/ml
Detection method:
Competitive Inhibition
Assay time:
2h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com