Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to double stranded DNA(dsDNA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to double stranded DNA(dsDNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain double stranded DNA(dsDNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of double stranded DNA(dsDNA) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
autoimmune disease
Species reactivity:
Rabbit
Alternative names:
dsDNA
Sample type:
serum, plasma, urine and other biological fluids
Detection range:
Sensitivity:
0.098ng/mL
Standard:
0.098ng/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com