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ELK Biotechnology

elk biotechnology

ELK biotechnology is a high-tech biological company focusing on the development and production of immune products and molecular biology products.
Their products cover around 8.000+ ELISA kits, 11.000+ antibodies, and multiple kinds of genome extraction reagents, molecular biology reagents and cell experiment related products.
ELK Biotechnology is involved in immunochemistry, tumor research, neurobiology, cell cycle, signal transduction among other fields.
Their products have strict R&D and quality inspection processes, for this reason each product has complete R&D, production and quality inspection records.

While developing, ELK Biotechnology always implements the concept of environmental protection in their production processes, and always takes scientific development and environmental protection as their company's mission. In line with the belief of providing scientists with the most cutting-edge and most cost-effective research tools, ELK Biotechnology will continue to devote themselves to providing high-quality scientific research products for scientific researchers and contributing to the development of human life sciences.

www.elkbiotech.com/

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General SIP(Sphingosine 1 Phosphate) ELISA Kit ELK9484



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Specifications

48T / 96T

Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sphingosine 1 Phosphate(SIP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate(SIP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate(SIP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;

Species reactivity:

General

Alternative names:

EDG3; LPB3; S1P3; S1PR3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; Sphingosine 1-phosphate receptor Edg-3

Sample type:

Tissue homogenates, cell lysates and other biological fluids

Detection range:

3.13-200 ng/mL

Sensitivity:

0.91 ng/mL

Standard:

200 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com